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Myosin light chain (phospho S20) Polyclonal Antibody, Cy5 Conjugated

Product Specifications

Background

Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.

Synonyms

LC20; MLC2; MRLC1; MYRL2; MLC-2C; Myosin regulatory light polypeptide 9; 20 kDa myosin light chain; Myosin RLC; Myosin regulatory light chain 2, smooth muscle isoform; Myosin regulatory light chain 9; Myosin regulatory light chain MRLC1; MYL9

Gene ID

10398

Swiss Prot

P24844

Modification Site

Ser20

Cellular Locus

Cytoplasm

Host

Rabbit

Cross Reactivity

Human, Mouse

Target

Myosin light chain (phospho S20)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Cy5

Source

KLH conjugated synthetic phosphopeptide derived from human Myosin Regulatory Light polypeptide 9 around the phosphorylation site of Ser20

Applications

WB, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

625,650nm/670nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

10398

Predicted Cross Reactivity

Rat, Dog, Cow, Sheep, Pig, Rabbit

Available Sizes

Curated Selection

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