Ephexin-1 (Tyr-87), Phosphospecific Antibody

Product Specifications

Background

The Eph family of receptor tyrosine kinases, and their ephrin ligands, are important for cell positioning and morphogenesis during development. EphA4 receptor can inhibit axon outgrowth and has roles in regulating axon projections during neural development. Eph signaling pathways require both receptor kinase activity and activation of Rho-GTPase guanine nucleotide-exchange factors (GEFs) . Ephexin-1 is a Dbl family GEF that may be important for regulating Rho GTPase activity downstream of EphA4/FGFR complexes. EphA4 activation leads to Src kinase phosphorylation of Tyr-87 in ephexin-1, which enhances activation of RhoA, but not Rac1 or Cdc42. In addition, ephexin-1 also binds FGFRs, and is phosphorylated at Tyr-87, as well as other tyrosine sites.

Synonyms

EphA, Eph, NGEF

Swiss Prot

Q8CHT1

Modification Site

Tyr-87

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat, Chicken

Target

Ephexin-1 (Tyr-87)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

Phospho-ephexin-1 (Tyr-87) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding tyrosine 87 in mouse ephexin-1.

Applications

Purification

Antigen Affinity purification

Dilution

WB (1:300-5000)

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Phosphorylation

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

The antibody detects an 80 kDa* band corresponding to ephexin-1 in Western blots of human A431 treated with pervanadate. This band is removed by alkaline phosphatase treatment. This sequence has significant homology to the conserved region in human and rat ephexin-1.