EEF2K (C-terminus) Antibody

Product Specifications

Background

Eukaryotic elongation factor 2 (eEF2) catalyzes the translocation of peptidyl-tRNA from the A site to the P site on the ribosome. eEF2 kinase (eEF2K) phosphorylates and inactivates eEF2, resulting in the inhibition of peptide-chain elongation. eEF2K is normally dependent on Ca2+ ions and calmodulin, and can be activated by PKA in response to elevated cAMP levels during cell stress- or starvation-related conditions. Regulation of eEF2K occurs through phosphorylation at multiple sites. Ser-78 phosphorylation is required for calmodulin binding and eEF2K activity, while phosphorylation of Ser-500 is required for Ca2+/calmodulin-independent kinase activity. Thr-348 is an autophosphorylation site that is required for kinase activity. Inhibitory phosphorylation may also regulate eEF2K, since phosphorylation at Ser-359 by SAPK4/p38delta causes inactivation of eEF2K. Thus, multisite phospho-regulation of eEF2K may be important for proper control of eEF2K activity and protein translation.

Synonyms

EEF-2, eEF-2K, CaMK-III, eukaryotic elongation factor

Swiss Prot

O00418

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat, Chicken, Xenopus

Target

EEF2K (C-terminus)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

EEF2K (C-terminus) synthetic peptide (coupled to carrier protein) corresponds to amino acids in the C-terminal region of human eEF2K.

Applications

WB, IP

Purification

Antigen Affinity purification

Dilution

WB (1:300-5000), IP (1-2ug)

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Unmodified

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

The antibody detects a 105 kDa* protein corresponding to the molecular mass of eEF2K on SDS-PAGE immunoblots of human A431, HeLa, and Jurkat cells. The antibody also detected eEF2K in immunoprecipitations in HeLa cells and immunofluorescent labeling in A431 cells. This sequence is well conserved in rat, mouse, chicken, and fish eEF2K, and has low homology to other proteins.