MDia3 (C-terminus) Antibody

Product Specifications

Background

Formins include several families of proteins that regulate actin cytoskeletal dynamics via two conserved formin homology domains, FH1 and FH2. Through cooperation of FH1 and FH2, formins construct actin-based structures comprising linear, unbranched filaments that are used in stress fibers, actin cables, microspikes, and contractile rings. A subgroup of the formins is the diaphanous (Dia) family, which includes mDia1 (Diap1), mDia2 (Diap3), and mDia3 (Diap2) . mDia3 is activated by Cdc42 and regulates the attachment of microtubules to the kinetochore during mitosis. Aurora B kinase phosphorylates Ser-196 and Thr-882 in vitro, and phosphorylation of Ser-196 increases during mitosis in vivo. A multisite mutant mDia3 with nonphosphorylatable T66A, S196A, S880A and T882A leads to misalignment of chromosomes at the metaphase plate. Thus, phosphorylation may be an important regulator of mDia3 activity during mitosis.

Synonyms

DIA3; DIAP2; DIAPH2; diaphanous formin-2; DRF2; POF2

Swiss Prot

Q9Z207

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat

Target

MDia3 (C-terminus)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

MDia3 synthetic peptide (coupled to KLH) corresponding to amino acid residues near the C-terminus of human mDia3 (Diap2) .

Applications

Purification

Antigen Affinity purification

Dilution

WB (1:300-5000)

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Unmodified

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

The antibody detects a 130 kDa* protein corresponding to the apparent molecular mass of mDia3 on SDS-PAGE immunoblots of human Jurkat and mouse C2C12 cells. This peptide sequence is highly conserved in rat and mouse mDia3, and has low homology to other formins.