MDia1 (N-terminal region) Antibody

Product Specifications

Background

Formins include several families of proteins that regulate actin cytoskeletal dynamics via two conserved formin homology domains, FH1 and FH2. Through cooperation of FH1 and FH2, formins construct actin-based structures comprising linear, unbranched filaments that are used in stress fibers, actin cables, microspikes, and contractile rings. A subgroup of the formins is the diaphanous (Dia) family, which includes mDia1 (Diap1), mDia2 (Diap3), and mDia3 (Diap2) . The mDia1 protein is activated by Rho and leads to ROCK-dependent stress fiber formation. Rho-activated mDia1 regulates whole antiserum response factor-dependent transcription. In cancers, mDia1 has been implicated in ras-mediated transformation, metastasis, and invasion. Thus, mDia1 is a Rho-activated formin with both cytoskeletal- and transcription-reguatling activities.

Synonyms

Dia1, Diaph1, DRF-1, Diap1, p140Dia, formin

Swiss Prot

O60610

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat

Target

MDia1 (N-terminal region)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

MDia1 synthetic peptide corresponding to amino acids from the N-terminal region of human mDia1 (Diap1) .

Applications

Purification

Antigen Affinity purification

Dilution

WB (1:300-5000)

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Unmodified

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

The antibody detects a 140 kDa* protein corresponding to the apparent molecular mass of mDia1 on SDS-PAGE immunoblots of human K562, human Jurkat, and mouse C2C12 cells. This peptide sequence is highly conserved in rat and mouse mDia1, and has low homology to other formins.