Xin Actin-binding Repeat-containing Protein 1 (XIRP1) (Ser295) Antibody

Product Specifications

Background

XIRP1 is a member of the Xin family of proteins, containing 16 Xin repeats. The intercalated disk protein Xin protects actin filaments from depolymerization, and is able to bundle actin filaments to interact with β-catenin, which is thought to stabilize actin-based cytoskeletons (Choi et al., 2007) . The evolutionary emergence of the Xin paralogs may have played a key role in the development of heart chambers with complete endothelial and myocardial layers (Grosskurth et al., 2008) . Loss of mXina (mouse Xin homolog) results in cardiac hypertrophy and cardiomyopathy (Gustafson-Wagner et al., 2007) . Phosphorylation of Ser-295 plays a key role in XIRP1 signaling, and therefore in cardiac hypertrophy, as it has been shown that acute pressure-overloaded hearts rapidly increased phosphorylation at the Ser-295 residue of XIRP1. (Chang et al., 2013) .

Synonyms

AI415219 antibody, Cardiomyopathy-associated protein 1 antibody, CMYA1 antibody, DKFZp451D042 antibody, DKFZp779C1255 antibody, DKFZp779C1947 antibody, MGC144145 antibody, MGC144146 antibody, Xin actin-binding repeat-containing protein 1 antibody, XIN antibody, Xirp1 antibody, XIRP1_HUMAN antibody

Gene ID

165904

Swiss Prot

O70373

Modification Site

Ser295

Host

Rabbit

Cross Reactivity

Mouse

Target

Xin Actin-binding Repeat-containing Protein 1 (XIRP1) (Ser295)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser295 of mouse XIRP, conjugated to keyhole limpet hemocyanin (KLH) .

Applications

Purification

Antigen Affinity purification from Pooled whole antiserum

Concentration

Lot Dependent

Dilution

WB (1:300-5000)

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylation

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

Specific for endogenous levels of the ~250 kDa and ~150 kDa splice variants of XIRP1 phosphorylated at Ser295. Immunolabeling is completely eliminated by treatment with λ-phosphatase.