Synapsin (Ser549) Antibody

Product Specifications

Background

Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996) . This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. The role of synapsin in synaptic plasticity and in synaptogenesis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002) . Ser-549 along with Ser-62 and Ser-67 are the sites of synapsin I that are phosphorylated by MAP kinase (Jovanovic et al., 1996) . Phosphorylation and subsequent dephosphorylation of this site is thought to play a key role in synaptic vesicle trafficking.

Synonyms

Brain protein 4.1 antibody, SYN 1 antibody, SYN 1a antibody, SYN 1b antibody, SYN I antibody, SYN1 antibody, SYN1_HUMAN antibody, SYN1a antibody, SYN1b antibody, Synapsin 1 antibody, Synapsin I antibody, Synapsin-1 antibody, Synapsin1 antibody, SynapsinI antibody, SYNI antibody

Gene ID

281510

Swiss Prot

P17599

Modification Site

Ser549

Host

Rabbit

Cross Reactivity

Mouse, Rat

Target

Synapsin (Ser549)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser549 of rat synapsin, conjugated to keyhole limpet hemocyanin (KLH) .

Applications

Purification

Antigen Affinity purification from Pooled whole antiserum

Concentration

Lot Dependent

Dilution

WB (1:300-5000)

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylation

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

Specific for endogenous levels of the ~78 kDa synapsin I doublet phosphorylated at Ser549. Immunolabeling is completely eliminated by treatment with λ-phosphatase.