What is the catalog number for Raf-1 (Ser642) Antibody?

The catalog number for Raf-1 (Ser642) Antibody is bs-70241R.

What sizes are available for Raf-1 (Ser642) Antibody?

Raf-1 (Ser642) Antibody is available in the following size(s): 100 µL.

Is Raf-1 (Ser642) Antibody in stock?

Raf-1 (Ser642) Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

How long will it take to receive this product if it’s custom made?

Lead times depend on the product type: custom polyclonal antibodies typically take 12-14 weeks, monoclonal antibodies 15-17 weeks, made-to-order recombinant proteins 4-8 weeks, and made-to-order recombinant antibodies 6-8 weeks. These are estimates and may shift due to production delays.

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

Raf-1 (Ser642) Antibody

Product Specifications

Background

The Ras pathway is a critical signal transduction cascade involved in regulating cellular proliferation, differentiation, survival, and oncogenic transformation. Members of the Raf serine/threonine kinase family are key intermediates in this cascade, functioning to relay signals from activated Ras to the downstream protein kinases MEK and ERK (Marshall, 1996) . Previous studies have shown that phosphorylation is required for Raf-1 activation (Dhillon and Kolch, 2002; Chong et al., 2003) . Recent work has demonstrated that phosphorylation also regulates the downregulation of Raf (Dougherty et al., 2005) with two sites participating: Ser-301 and Ser-642.

Synonyms

C Raf antibody, C-raf antibody, C-Raf proto-oncogene serine/threonine kinase antibody, CMD1NN antibody, Craf 1 transforming gene antibody, cRaf antibody, Craf1 transforming gene antibody, EC 2.7.11.1 antibody, kinase Raf1 antibody, Murine sarcoma 3611 oncogene 1 antibody, NS5 antibody, Oncogene MIL antibody, Oncogene RAF1 antibody, OTTHUMP00000160218 antibody, OTTHUMP00000207813 antibody, OTTHUMP00000209389 antibody, Protein kinase raf 1 antibody, Proto-oncogene c-RAF antibody, Raf 1 antibody, Raf 1 proto oncogene serine/threonine kinase antibody, RAF antibody, Raf proto oncogene serine/threonine protein kinase antibody, RAF proto-oncogene serine/threonine-protein kinase antibody, RAF-1 antibody, RAF1 antibody, RAF1_HUMAN antibody, Similar to murine leukemia viral (V-raf-1) oncogene homolog 1 antibody, TRANSFORMING REPLICATION-DEFECTIVE MURINE RETROVIRUS 3611-MSV antibody, v raf 1 murine leukemia viral oncogene homolog 1 antibody, v-raf murine sarcoma viral oncogene homolog 1 antibody, v-raf-1 murine leukemia viral oncogene-like protein 1 antibody, vraf1 murine leukemia viral oncogene homolog 1 antibody

Gene ID

24703

Swiss Prot

P11365

Modification Site

Ser642

Host

Rabbit

Cross Reactivity

Human, Rat

Target

Raf-1 (Ser642)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser642 of rat Raf-1, conjugated to keyhole limpet hemocyanin (KLH) .

Applications

Purification

Antigen Affinity purification from Pooled whole antiserum

Concentration

Lot Dependent

Dilution

WB (1:300-5000)

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylation

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

Specific for endogenous levels of the ~74 kDa Raf-1 protein phosphorylated at Ser642. Immunolabeling is completely eliminated by treatment with λ-phosphatase.