PTPH1 (Ser459) Antibody

Product Specifications

Background

Protein-tyrosine Phosphatase H1 (PTPH1) has recently been identified as a specific p38γ MAPK phosphatase which is mediated through PDZ interaction (Hou et al., 2010) . Ras has been demonstrated to increase both p38γ and PTPH1 protein expression, and there is a coupling of increased p38γ and PTPH1 protein expression in primary colon cancer tissues (Hou et al., 2010) . Phosphorylation of PTPH1 at Ser-459 leads to PTPH1 stabilization, which plays an important role in Ras oncogenesis and stress response (Hou et al., 2012) . Additionally, phosphorylation of PTPH1 Ser-459 reveals a novel mechanism by which MAPK signals through PTPH1 to regulate cellular response (Hou et al., 2012) .

Synonyms

Cytoskeletal-associated protein tyrosine phosphatase antibody, protein tyrosine phosphatase H1 antibody, protein tyrosine phosphatase non-receptor type 3 antibody, Protein-tyrosine phosphatase H1 antibody, PTN3_HUMAN antibody, PTP-H1 antibody, PTPH1 antibody, PTPN3 antibody, Tyrosine-protein phosphatase non-receptor type 3 antibody

Gene ID

5774

Swiss Prot

P26045

Modification Site

Ser459

Host

Rabbit

Cross Reactivity

Human, Mouse

Target

PTPH1 (Ser459)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser459 of human PTPH1, conjugated to keyhole limpet hemocyanin (KLH) .

Applications

Purification

Antigen Affinity purification from Pooled whole antiserum

Concentration

Lot Dependent

Dilution

WB (1:300-5000)

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylation

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

Specific for endogenous levels of the ~104 kDa PTPH1 protein phosphorylated at Ser459. The immunolabeling is completely eliminated by treatment with λ-phosphatase.