Polo-Like Kinase Kinase (Ser482,486,490) Antibody
Selected Variant
bs-70225R
Size: 100 µL
Product Specifications
Background
Considerable evidence indicates that a polo-like kinase (PLK) plays an important role in cell cycle regulation. PLK is also required for bipolar spindle formation, activation of the anaphase-promoting complex/cyclosome, and cytokinesis. Recent work led to the identification of a PLKK that is thought to be responsible for activation of PLK. Recent work (Erikson, et al., 2004) has shown that PLKK is in turn activated by phosphorylation at three sites (Ser-482, Ser-486 and Ser-490) . Thus activation of PLK is thought to involve a kinase cascade involving the phosphorylation of Ser-482,486,490 in PLKK.
Synonyms
Polo like kinase kinase 1 antibody, Serine/threonine kinase 10 antibody, stk10 a antibody, xPlkk1 antibody
Gene ID
380481
Swiss Prot
B7ZR30
Modification Site
Ser482,486,490
Host
Rabbit
Cross Reactivity
Rat, Xenopus
Target
Polo-Like Kinase Kinase (Ser482,486,490)
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Source
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser482/486/490 of Xenopus polo-like kinase kinase (PLKK), conjugated to keyhole limpet hemocyanin (KLH) .
Applications
WB
Purification
Antigen Affinity purification from Pooled whole antiserum
Concentration
Lot Dependent
Dilution
WB (1:300-5000)
Buffer
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Modification
Phosphorylation
Storage Conditions
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Specificity
Specific for endogenous levels of the ~120k PLKK protein phosphorylated at Ser482,486,490.
Gene ID URL
380481
Predicted Cross Reactivity
Human, Mouse, Zebrafish
Price
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