Polo-Like Kinase Kinase (Ser482,486,490) Antibody

Product Specifications

Background

Considerable evidence indicates that a polo-like kinase (PLK) plays an important role in cell cycle regulation. PLK is also required for bipolar spindle formation, activation of the anaphase-promoting complex/cyclosome, and cytokinesis. Recent work led to the identification of a PLKK that is thought to be responsible for activation of PLK. Recent work (Erikson, et al., 2004) has shown that PLKK is in turn activated by phosphorylation at three sites (Ser-482, Ser-486 and Ser-490) . Thus activation of PLK is thought to involve a kinase cascade involving the phosphorylation of Ser-482,486,490 in PLKK.

Synonyms

Polo like kinase kinase 1 antibody, Serine/threonine kinase 10 antibody, stk10 a antibody, xPlkk1 antibody

Gene ID

380481

Swiss Prot

B7ZR30

Modification Site

Ser482,486,490

Host

Rabbit

Cross Reactivity

Rat, Xenopus

Target

Polo-Like Kinase Kinase (Ser482,486,490)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser482/486/490 of Xenopus polo-like kinase kinase (PLKK), conjugated to keyhole limpet hemocyanin (KLH) .

Applications

Purification

Antigen Affinity purification from Pooled whole antiserum

Concentration

Lot Dependent

Dilution

WB (1:300-5000)

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylation

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.