Polo-Like Kinase (Thr210) Antibody

Product Specifications

Background

Polo-like kinases are important regulators of cell cycle progression. PLK1 is a highly conserved Ser/Thr kinase that has essential roles in the formation of mitotic bipolar spindles (van Vugt et al., 2004) . Deregulated expression of PLK’s is detected in many types of cancer and associated with oncogenesis (Takei et al., 2005) . It has been proposed that PLK1 function is altered at different stages of mitosis through consecutive phosphorylation events at Ser-137 and Thr-210 (van de Weerdt et al., 2005) .

Synonyms

Cell cycle regulated protein kinase antibody, PLK 1 antibody, PLK antibody, PLK-1 antibody, plk1 antibody, PLK1_HUMAN antibody, Polo like kinase 1 antibody, Polo-like kinase 1 antibody, Serine/threonine protein kinase 13 antibody, Serine/threonine protein kinase PLK1 antibody, Serine/threonine-protein kinase 13 antibody, Serine/threonine-protein kinase PLK1 antibody, STPK 13 antibody, STPK13 antibody

Gene ID

5347

Swiss Prot

P53350

Modification Site

Thr210

Host

Rabbit

Cross Reactivity

Human, Rat

Target

Polo-Like Kinase (Thr210)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr210 of human PLK1, conjugated to keyhole limpet hemocyanin (KLH) .

Applications

Purification

Antigen Affinity purification from Pooled whole antiserum

Concentration

Lot Dependent

Dilution

WB (1:300-5000)

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylation

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

Specific for endogenous levels of the ~66 kDa PLK phosphorylated at Thr210. The immunolabeling is completely eliminated by treatment with λ-phosphatase.