Polo-Like Kinase (Thr210) Antibody
Product Specifications
Background
Polo-like kinases are important regulators of cell cycle progression. PLK1 is a highly conserved Ser/Thr kinase that has essential roles in the formation of mitotic bipolar spindles (van Vugt et al., 2004) . Deregulated expression of PLK’s is detected in many types of cancer and associated with oncogenesis (Takei et al., 2005) . It has been proposed that PLK1 function is altered at different stages of mitosis through consecutive phosphorylation events at Ser-137 and Thr-210 (van de Weerdt et al., 2005) .
Synonyms
Cell cycle regulated protein kinase antibody, PLK 1 antibody, PLK antibody, PLK-1 antibody, plk1 antibody, PLK1_HUMAN antibody, Polo like kinase 1 antibody, Polo-like kinase 1 antibody, Serine/threonine protein kinase 13 antibody, Serine/threonine protein kinase PLK1 antibody, Serine/threonine-protein kinase 13 antibody, Serine/threonine-protein kinase PLK1 antibody, STPK 13 antibody, STPK13 antibody
Gene ID
5347
Swiss Prot
P53350
Modification Site
Thr210
Host
Rabbit
Cross Reactivity
Human, Rat
Target
Polo-Like Kinase (Thr210)
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Source
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr210 of human PLK1, conjugated to keyhole limpet hemocyanin (KLH) .
Applications
WB
Purification
Antigen Affinity purification from Pooled whole antiserum
Concentration
Lot Dependent
Dilution
WB (1:300-5000)
Buffer
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Modification
Phosphorylation
Storage Conditions
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Specificity
Specific for endogenous levels of the ~66 kDa PLK phosphorylated at Thr210. The immunolabeling is completely eliminated by treatment with λ-phosphatase.
Gene ID URL
5347
Predicted Cross Reactivity
Mouse, Dog, Bovine, Zebrafish, Xenopus, Non-Human Primate
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