PanK4 (Thr63) Antibody

Product Specifications

Background

Pantothenate kinase, PanK, is a vital regulatory enzyme for coenzyme A (CoA) biosynthesis, phosphorylating pantothenate (vitamin B5) to 4’-phosphopantothenate, then quickly transforming to CoA which is an essential component for fatty acid metabolism (Abiko, Y, 1967) . There are 4 members of the PanK family, located on chromosomes 10q23.31, 20p13, 5q35, and 1p36.32 (Zhou et al, 2001) . PanK1 is predominantly in heart, liver, and kidney. PanK2 is expressed ubiquitously, with higher levels in retinal and infant basal ganglia. PanK3 has high levels in liver, while PanK4 is expressed ubiquitously with its highest levels found in muscle (Zhou et al, 2001) . Additionally, PanK4 has been shown to regulate Pkm2 activity affecting glucose metabolism (Li et al, 2005) . There have been several phospho-serine, threonine, and tyrosine sites identified within PanK4, the role of each one has yet to be determined.

Synonyms

DKFZp547M242 antibody, FLJ10782 antibody, hPanK 4 antibody, hPanK4 antibody, PANK 4 antibody, Pantothenate kinase 4 antibody, Pantothenic acid kinase antibody

Gene ID

269614

Swiss Prot

Q80YV4

Modification Site

Thr63

Host

Rabbit

Cross Reactivity

Mouse

Target

PanK4 (Thr63)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr63 of mouse PanK4, conjugated to keyhole limpet hemocyanin (KLH) .

Applications

Purification

Antigen Affinity purification from Pooled whole antiserum

Concentration

Lot Dependent

Dilution

WB (1:300-5000)

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylation

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.