P53 (Ser392) Antibody

Product Specifications

Background

P53 has a well established role in blocking the proliferative action of damaged cells and acting in essence as an anticancer agent (Sharpless and DePinho, 2002; Yin et al., 1992) . It has been called the guardian of the genome (Lane, 1992) . Phosphorylation of Ser-392 in p53 is associated with formation of human tumors (Saito et al., 2003; Pise-Masison et al., 1998; Kim et al., 2004) . In addition, p53 has been linked to effects of aging and oxidative stress (Sharpless and DePinho, 2002) . An increase in p53 has also been linked to deficits in LTP and learning and memory (Jiang et al., 1998)

Synonyms

Antigen NY-CO-13 antibody, BCC7 antibody, Cellular tumor antigen p53 antibody, FLJ92943 antibody, LFS1 antibody, Mutant tumor protein 53 antibody, p53 antibody, p53 tumor suppressor antibody, P53_HUMAN antibody, Phosphoprotein p53 antibody, Tp53 antibody, Transformation related protein 53 antibody, TRP53 antibody, Tumor protein 53 antibody, Tumor protein p53 antibody, Tumor suppressor p53 antibody

Gene ID

7157

Swiss Prot

P04637

Modification Site

Ser392

Host

Rabbit

Cross Reactivity

Human, Rat

Target

P53 (Ser392)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser392 of human p53, conjugated to keyhole limpet hemocyanin (KLH) .

Applications

Purification

Antigen Affinity purification from Pooled whole antiserum

Concentration

Lot Dependent

Dilution

WB (1:300-5000)

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylation

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

Specific for endogenous levels of the ~53 kDa p53 protein phosphorylated at Ser392 ~53 kDa p53 protein phosphorylated at Ser392. The immunolabeling is completely eliminated by treatment with λ-phosphatase.