NMDA NR2B Subunit (Tyr1252) Antibody
Product Specifications
Background
The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002) . The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993) . Phosphorylation of Tyr-1252 is thought to potentiate NMDA receptor-dependent influx of calcium (Takasu et al., 2002) .
Synonyms
EPND antibody, FESD antibody, GluN2A antibody, Glutamate [NMDA] receptor subunit epsilon-1 antibody, Glutamate receptor antibody, Glutamate receptor ionotropic N methyl D aspartate 2A antibody, GRIN 2A antibody, GRIN2A antibody, hNR2A antibody, LKS antibody, N methyl D aspartate receptor channel subunit epsilon 1 antibody, N Methyl D Aspartate Receptor Subtype 2A antibody, N methyl D aspartate receptor subunit 2A antibody, N-methyl D-aspartate receptor subtype 2A antibody, NMDA receptor subtype 2A antibody, NMDAR 2A antibody, NMDAR2A antibody, NMDE1_HUMAN antibody, NR2A antibody, OTTHUMP00000160135 antibody, OTTHUMP00000174531 antibody
Gene ID
24410
Swiss Prot
Q00960
Modification Site
Tyr1252
Host
Rabbit
Cross Reactivity
Mouse, Rat
Target
NMDA NR2B Subunit (Tyr1252)
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Source
Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1252 of the NR2B subunit of the rat NMDA receptor, conjugated to keyhole limpet hemocyanin (KLH) .
Applications
WB, IHC
Purification
Antigen Affinity purification from Pooled whole antiserum
Concentration
Lot Dependent
Dilution
WB (1:300-5000), IHC ()
Buffer
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Modification
Phosphorylation
Storage Conditions
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Product Datasheet
https://www.biossantibodies.com/datasheets/bs-70194R
Specificity
Specific for endogenous levels of the ~180 kDa NMDAR NR2B-subunit protein phosphorylated at Tyr1252 . Immunolabeling is completely eliminated by treatment with λ-phosphatase. May also show slight reactivity with Tyr1246 of NR2A.
Gene ID URL
24410
Predicted Cross Reactivity
Human, Dog, Bovine, Chicken, Zebrafish, Non-Human Primate
Curated Selection
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