NMDA NR1 Subunit, Splice Variant C2' Antibody

The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002) . Increased membrane surface expression of the NMDAR, NR1-Subunit has been associated with synaptic plasticity (Grosshans et al., 2002) . There are a number of different splice variants of the NR1-Subunit (Foldes et al., 1994; Zukin and Bennett, 1995) . Differential splicing of three exons in the NR1-Subunit generates up to eight NR1-Subunit splice variants and 7 of these have been identified in cDNA libraries. These exons encode a 21 amino acid N-terminal domain (N1) and adjacent sequences in the C-terminus (C1 and C2) . Splicing out the C2 cassette eliminates the first stop codon and produces a new reading frame that generates a new sequence of 22 amino acids (C2') . Considerable attention has been focused on the distribution and expression of these splice variants that may affect the functional properties and regulation of the NMDAR.

GluN1 antibody, Glutamate [NMDA] receptor subunit zeta-1 antibody, Glutamate receptor ionotropic N methyl D aspartate 1 antibody, Glutamate receptor ionotropic N-methyl-D aspartate subunit 1 antibody, glutamate receptor ionotropic NMDA 1 antibody, Grin1 antibody, MRD8 antibody, N methyl D aspartate receptor antibody, N methyl D aspartate receptor channel subunit zeta 1 antibody, N methyl D aspartate receptor subunit NR1 antibody, N-methyl-D-aspartate receptor subunit NR1 antibody, NMD-R1 antibody, NMDA 1 antibody, NMDA R1 antibody, NMDAR1 antibody, NMDA receptor 1 antibody, NMDA1 antibody, NMDAR antibody, NMDZ1_HUMAN antibody, NR1 antibody

Synthetic peptide corresponding to amino acid residues specific to the NR1 subunit, C2' splice variant insert of the rat NMDA receptor, conjugated to keyhole limpet hemocyanin (KLH) .

Reconstitute in 50 ul phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution it is recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Specific for endogenous levels of the ~120 kDa NR1 subunit of the NMDA receptor containing the C2’ splice variant insert. Does not recognize the NR1 subunits of the NMDA receptor that do not contain the C2’ insert.