NCC (Thiazide sensitive NaCl cotransporter) (Thr53) Antibody

Product Specifications

Background

The thiazide-sensitive sodium chloride cotransporter, NCC, is the major NaCl transport protein in the distal convoluted tubule (DCT) and plays an important role in maintaining blood pressure (Rosenbaek et al., 2014, Feng et al., 2015) . Phosphorylation of NCC at Thr-53, Thr-58, and Ser-71 is an essential mediator of NCC function (Rosenbaek et al., 2014) . NCC is constitutively cycled to the plasma membrane, and upon stimulation, it can be phosphorylated to both increase NCC activity and decrease NCC endocytosis, together increasing NaCl transport in the DCT (Feng et al., 2015) .

Synonyms

FLJ96318 antibody, Na Cl cotransporter antibody, Na Cl symporter antibody, Na-Cl symporter antibody, NaCl electroneutral thiazide sensitive cotransporter antibody, NCCT antibody, SLC12A3 antibody, S12A3_HUMAN antibody, slc12a3 antibody, Solute carrier family 12 (sodium/chloride transporters) member 3 antibody, Solute carrier family 12 member 3 antibody, Thiazide sensitive Na Cl cotransporter antibody, Thiazide sensitive sodium chloride cotransporter antibody, Thiazide-sensitive sodium-chloride cotransporter antibody, TSC antibody

Gene ID

20497

Swiss Prot

P59158

Modification Site

Thr53

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat

Target

NCC (Thiazide sensitive NaCl cotransporter)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr53 of mouse NCC, conjugated to keyhole limpet hemocyanin (KLH) .

Applications

WB, IHC

Purification

Antigen Affinity purification from Pooled whole antiserum

Concentration

Lot Dependent

Dilution

WB (1:300-5000), IHC ()

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylation

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

Specific for endogenous levels of the ~160 kDa NCC protein phosphorylated at Thr53. Band of interest smearing likely due to glycosylation. Immunolabeling is completely eliminated by treatment with l-phosphatase.