Metabotropic Glutamate Receptor 7 (Ser862) Antibody

Product Specifications

Background

Metabotropic glutamate receptors (mGluRs) are key receptors in the modulation of excitatory synaptic transmission in the central nervous system. They are implicated in many forms of neural plasticity as well as learning and memory and drug abuse (Bhattacharya et al., 2004; Francesconi et al., 2004; Wilson and Nicoll, 2001) . The mGluRs are divided into three groups based on sequence identity and pharmacological properties: group I (mGluR1 and mGluR5) are localized in the perisynaptic region of the postsynaptic membrane, whereas group II (mGlur2 and mGluR3) and group III (mGluR4,6,7 and 8) are localized predominantly at presynaptic terminals. PKC phosphorylation of Ser-862 on mGluR7 has been shown to be critical for stabilizing receptor surface expression and promoting binding to the synaptic PDZ-domain-containing protein PICK1 (Suh et al., 2008) .

Synonyms

FLJ40498 antibody, GLUR7 antibody, Glutamate receptor metabotropic 7 antibody, GPRC1G antibody, GRM7 antibody, GRM7_HUMAN antibody, Metabotropic glutamate receptor 7 antibody, MGLU7 antibody, mGluR7 antibody, OTTHUMP00000206961 antibody, OTTHUMP00000214674 antibody, OTTHUMP00000214675 antibody

Gene ID

81672

Swiss Prot

P35400

Modification Site

Ser862

Host

Rabbit

Cross Reactivity

Mouse, Rat

Target

Metabotropic Glutamate Receptor 7 (Ser862)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser862 of rat mGluR7, conjugated to keyhole limpet hemocyanin (KLH) .

Applications

Purification

Antigen Affinity purification from Pooled whole antiserum

Concentration

Lot Dependent

Dilution

WB (1:300-5000)

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylation

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

Specific for the ~102 kDa mGluR7 protein phosphorylated at Ser862. Immunolabeling is blocked by preadsorption of antibody with the phospho-peptide used as antigen but not by the corresponding dephosphopeptide.