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MEK5 (Ser311, Thr315) Antibody

Product Specifications

Background

MEK5 (also known as MKK5) is a dual specificity serine/threonine protein kinase belonging to the MAP kinase kinase family. MEK5 has been shown to specifically activate ERK5 (Zhou et al., 1995) whereas MEK5 itself is regulated by MEKK3 (Chao et al., 1999) . An important link between MEK5 and metastatic prostrate cancer has been demonstrated (Mehta et.al., 2003) . Dual phosphorylation of Ser-311 and Thr-315 have been implicated in cell proliferation (Cameron et al., 2004) .

Synonyms

Dual specificity mitogen activated protein kinase kinase 5 antibody, Dual specificity mitogen-activated protein kinase kinase 5 antibody, EC 2.7.12.2 antibody, HsT17454 antibody, MAP kinase kinase 5 antibody, MAP kinase kinase MEK5b antibody, MAP2K5 antibody, MAPK/ERK kinase 5 antibody, MAPKK 5 antibody, MAPKK5 antibody, MEK 5 antibody, mitogen-activated protein kinase kinase 5 antibody, MKK5 antibody, MP2K5_HUMAN antibody, PRKMK5 antibody, Protein kinase mitogen-activated kinase 5 antibody, SAPKK5 antibody, SKK5 antibody

Gene ID

31068

Swiss Prot

Q62862

Modification Site

Ser311, Thr315

Host

Rabbit

Cross Reactivity

Rat

Target

MEK5 (Ser311, Thr315)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser311/Thr315 of rat MEK5, conjugated to keyhole limpet hemocyanin (KLH) .

Applications

WB

Purification

Antigen Affinity purification from Pooled whole antiserum

Concentration

Lot Dependent

Dilution

WB (1:300-5000)

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylation

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Datasheet

https://www.biossantibodies.com/datasheets/bs-70155R

Specificity

Specific for endogenous levels of the ~49 kDa MEK5 protein phosphorylated at Ser311, Thr315. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Gene ID URL

31068

Predicted Cross Reactivity

Human, Mouse, Dog, Bovine, Chicken, Zebrafish, Xenopus, Non-Human Primate

Curated Selection

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