MEK1/2 (Ser218,222) Antibody

Product Specifications

Background

MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003) . This pathway also plays a key role in synaptic plasticity in the brain (Adams and Sweatt, 2002) . Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992) .

Synonyms

AA589381 antibody, CFC3 antibody, Dual specificity mitogen-activated protein kinase kinase 1 antibody, Dual specificity mitogen-activated protein kinase kinase 2 antibody, EC 2.7.12.2 antibody, ERK activator kinase 1 antibody, ERK activator kinase 2 antibody, FLJ26075 antibody, MAP kinase kinase 1 antibody, MAP kinase kinase 2 antibody, MAP2K1 antibody, MAP2K2 antibody, MAPK/ERK kinase 1 antibody, MAPK/ERK kinase 2 antibody, MAPKK 1 antibody, MAPKK1 antibody, MAPKK2 antibody, MEK 1 antibody, MEK1 antibody, MEKK1 antibody, Mitogen activated protein kinase kinase 1 antibody, Mitogen activated protein kinase kinase 2 antibody, Mitogen-activated protein kinase kinase 2, p45 antibody, MK2 antibody, MKK 1 antibody, MKK 2 antibody, MKK1 antibody, MKK2 antibody, MP2K1_HUMAN antibody, PRKMK 1 antibody, PRKMK 2 antibody, Prkmk1 antibody, Prkmk2 antibody, protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1) antibody, Protein kinase, mitogen-activated, kinase 1 antibody, Protein kinase, mitogen-activated, kinase 2 antibody

Gene ID

5604

Swiss Prot

Q02750

Modification Site

Ser218,222

Host

Rabbit

Cross Reactivity

Human, Mouse

Target

MEK1/2 (Ser218,222)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser218/222 of human MEK1/2, conjugated to keyhole limpet hemocyanin (KLH) .

Applications

Purification

Antigen Affinity purification from Pooled whole antiserum

Concentration

Lot dependent

Dilution

WB (1:300-5000)

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylation

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

Specific for endogenous levels of the ~45 kDa MEK 1/2 protein phosphorylated at Ser218/222. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide. Note: The phosphosites in human MEK 1/2 are Ser218 and Ser222; the phosphosites in mouse MEK 1/2 are Ser217 and Ser221.