MEF2C (Ser222) Antibody

Product Specifications

Background

MEF2C, also known as MADS box transcription enhancer factor 2, polypeptide C, is one of 4 MEF2 (myocyte enhancer factor 2) transcription factors that encode proteins for development of skeletal muscle and brain proliferation and differentiation (McDermott et al, 1993), along with regulating stress-response during cardiac hypertrophy in mammals (Wu et al, 2015) . Phosphorylation of MEF2C at serine 59 has been shown to be negatively regulated by integrin-linked kinase (ILK) (Dong et al, 2015) . Recently, high MEF2C expression has been associated with a subset of acute myeloid leukemia (AML) patients with adverse-risk disease features and poor outcomes (Laszlo et al, 2015) . Phosphorylation of MEF2C at serine 222 may play a key role in MEF2C signaling.

Synonyms

C5DELq14.3 antibody, DEL5q14.3 antibody, MADS box transcription enhancer factor 2 polypeptide C (myocyte enhancer factor 2C) antibody, MADS box transcription enhancer factor 2 polypeptide C antibody, MEF2C antibody, MEF2C_HUMAN antibody, Myocyte enhancer factor 2C antibody, Myocyte specific enhancer factor 2C antibody, Myocyte-specific enhancer factor 2C antibody, OTTHUMP00000222409 antibody, Similar to MADS box transcription enhancer factor 2 polypeptide C antibody

Gene ID

4208

Swiss Prot

Q06413

Modification Site

Ser222

Host

Rabbit

Cross Reactivity

Human

Target

MEF2C (Ser222)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser222 of human MEF2C, conjugated to keyhole limpet hemocyanin (KLH) .

Applications

Purification

Antigen Affinity purification from Pooled whole antiserum

Concentration

Lot Dependent

Dilution

WB (1:300-5000)

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylation

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

Specific for endogenous levels of the ~51 kDa MEF2C protein phosphorylated at Ser222. Immunolabeling is completely eliminated by treatment with λ-phosphatase.