Insulin Receptor (Thr1160) Antibody

The insulin receptor (IR) is a well-studied receptor tyrosine kinase composed of two α subunits, responsible for the extracellular insulin binding site, and two β subunits, responsible for intracellular protein kinase activity (Endemann et al., 1990, Chiu et al., 2010) . The binding of insulin to the α subunits activates the intrinsic kinase activity located in the β subunits and subsequently initiates a cascade of phosphorylation events causing major conformational changes in the activation loop of the kinase domain, which lead to different biological functions (Chiu et al., 2010) . It has been hypothesized that Thr-1160 phosphorylation affects or is affected by Tyr-1158/62/63 phosphorylation and that the conformation of Thr-1160 and pThr-1160 is likely to be affected by the phosphorylation status of the surrounding tyrosines.

CD220 antibody, HHF5 antibody, human insulin receptor antibody, Insr antibody, INSR_HUMAN antibody, Insulin receptor subunit beta antibody, IR 1 antibody, IR antibody, IR-1 antibody, IR1 antibody

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr1160 of human insulin receptor, conjugated to keyhole limpet hemocyanin (KLH) .

Specific for endogenous levels of the ~95 kDa Insulin receptor phosphorylated at Thr1160. Immunolabeling is completely eliminated with λ-phosphatase treatment.