Glycogen synthase kinase 3 B (GSK3B) (Ser9) Antibody

Product Specifications

Background

Glycogen synthase kinase 3 (GSK3) is a serine/threonine kinase that is involved in the regulation of many signaling pathways. To date, 2 isoforms have been identified: GSK3α and GSK3β. Specifically, GSK3β has been shown to play a key inhibitory role in both the insulin and Wnt signaling pathways (Papkoff and Aikawa 1998) . It has been suggested that Ser-9 phosphorylation underlies the inhibition of GSK3β by insulin (Sutherland et al., 1993) .

Synonyms

Glycogen Synthase Kinase 3 Beta antibody, Glycogen synthase kinase-3 beta antibody, GSK 3 beta antibody, GSK-3 beta antibody, GSK3B antibody, GSK3B_HUMAN antibody, GSK3beta isoform antibody, Serine/threonine-protein kinase GSK3B antibody

Gene ID

84027

Swiss Prot

P18266

Modification Site

Ser9

Host

Rabbit

Cross Reactivity

Mouse, Rat

Target

Glycogen synthase kinase 3 B (GSK3B) (Ser9)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser9 of rat GSK3β, conjugated to keyhole limpet hemocyanin (KLH) .

Applications

Purification

Antigen Affinity purification from Pooled whole antiserum

Concentration

Lot Dependent

Dilution

WB (1:300-5000)

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylation

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

Specific for the ~46 kDa GSK3β protein phosphorylated at Ser9. Also weakly labels the ~51 kDa GSK3β band due to the high degree of homology between the 2 subunits. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.