GluR1-Subunit (Ser845) Antibody

Product Specifications

Background

The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by α-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR) . The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinänen et al., 1990; Hollmann and Heinemann, 1994) . The GluR1 subunit is widely expressed throughout the nervous system. Phosphorylation of Ser-845 on GluR1 is thought to be mediated by PKA and phosphorylation of this site increases the conductance of the AMPAR (Roche et al., 1996; Banke et al., 2000) . In addition, phosphorylation of this site has been linked to synaptic plasticity as well as learning and memory (Lee at al., 2003; Esteban at al., 2003) .

Synonyms

GLUR 1 antibody, GLUR A antibody, AMPA 1 antibody, AMPA selective glutamate receptor 1 antibody, AMPA-selective glutamate receptor 1 antibody, GluA1 antibody, GLUH1 antibody, GluR K1 antibody, GluR-1 antibody, GluR-A antibody, GluR-K1 antibody, GLUR1 antibody, GLURA antibody, GluRK1 antibody, Glutamate receptor 1 antibody, Glutamate receptor ionotropic AMPA 1 antibody, Glutamate receptor ionotropic antibody, Glutamate receptor, ionotropic, AMPA 1 antibody, Gria1 antibody, GRIA1_HUMAN antibody, HBGR1 antibody, MGC133252 antibody, OTTHUMP00000160643 antibody, OTTHUMP00000165781 antibody, THUMP00000224241 antibody, OTTHUMP00000224242 antibody, OTTHUMP00000224243 antibody

Gene ID

50592

Swiss Prot

P19490

Modification Site

Ser845

Host

Rabbit

Cross Reactivity

Mouse, Rat

Target

GluR1-Subunit (Ser845)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser845 of rat GluR1, conjugated to keyhole limpet hemocyanin (KLH) .

Applications

WB, IHC

Purification

Antigen Affinity purification from Pooled whole antiserum

Concentration

Lot Dependent

Dilution

WB (1:300-5000), IHC ()

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylation

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

Specific for endogenous levels of the ~100 kDa GluR1 protein phosphorylated at Ser845. Immunolabeling is completely eliminated by treatment with λ-phosphatase.