GABA Transporter (GAT) 1 Antibody

Product Specifications

Background

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl− channel associated with the GABA-A receptor (GABA-A-R) subtype. GABA plasma membrane transporters (GATs) influence synaptic neurotransmission by high affinity uptake and release of GABA. To date, four distinct GABA transporters have been identified: GAT-1, GAT-2, GAT-3, and BGT-1. GAT-1, the most abundant of the transporters, is found predominantly in neurons, but also in some specialized glia (Minelli et al., 1995) . GAT-1 is thought to play a key role in epileptogenesis (Zhao et al. 2003) .

Synonyms

GABATHG antibody, GABATR antibody, GABT 1 antibody, GABT1 antibody, GAT-1 antibody, GAT1 antibody, SC6A1_HUMAN antibody, Slc6a1 antibody, Sodium and chloride dependent GABA transporter 1 antibody, Sodium- and chloride-dependent GABA transporter 1 antibody, Solute carrier family 6 (neurotransmitter transporter GABA) member 1 antibody, Solute carrier family 6 member 1 antibody

Gene ID

79212

Swiss Prot

P23978

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat, Non-Human Primate

Target

GABA Transporter (GAT) 1

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

Synthetic peptide corresponding to amino acid residues from the C-terminal region of rat GAT1, conjugated to keyhole limpet hemocyanin (KLH) .

Applications

WB, IHC

Purification

Affinity purification

Concentration

Lot Dependent

Dilution

WB (1:300-5000), IHC ()

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Unmodified

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

Specific for endogenous levels of the ~67 kDa GAT-1 protein. Immunolabeling is blocked by pre-adsorption of antibody with the peptide used to generate the antibody.