Fractin Antibody

Product Specifications

Background

Fractin (fragment of actin) is a caspase-specific cleavage product of actin and serves as a novel marker of apoptosis-related events. The antibody has been shown to detect the processes and cell bodies of degenerating neurons and plaque-associated microglia in Alzheimer’s disease (Yang et al., 1998) . It has recently been reported that Fractin may have a functional role in apoptotic signaling in oligodendrocytes (Schulz, R., et al., Glia, 2009, in press) .

Synonyms

Fragment of actin, actin cleavage product

Host

Rabbit

Cross Reactivity

Human, Rat

Target

Fractin

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

Synthetic peptide corresponding to amino acid residues from the C-terminal region of the 32 kDa actin fragment.

Applications

WB, IHC

Purification

Neat whole antiserum

Concentration

Lot Dependent

Dilution

WB (1:300-5000), IHC ()

Buffer

Neat whole antiserum

Modification

Unmodified

Storage Conditions

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Specificity

Specific for endogenous levels of the ~32 kDa fractin protein. Shows no reactivity toward intact actin. There is often a ladder of smaller bands in cells or culture or in vivo preparations due to further degradation by other proteases. In IHC the antibody detects the processes and cell bodies of degenerating neurons and plaque-associated microglia in Alzheimer's disease.