Elk-1 (Ser383) Antibody

Product Specifications

Background

Elk-1 is a transcription factor involved in mediating gene transcription in response to growth factors (Hill and Treisman, 1995) . Elk-1 is thought to be phosphorylated by MAP kinase at Ser-383 and phosphorylation at this site is essential for the transcriptional activity of Elk-1 (Li et al., 2003) . Phosphorylation of Elk-1 has also been implicated in synaptic plasticity in the adult hippocampus (Thiels et al., 2002) .

Synonyms

ELK 1 antibody, Elk1 antibody, ELK1 member of ETS oncogene family antibody, ELK1 protein antibody, ELK1, ETS transcription factor antibody, ELK1_HUMAN antibody, ELK2 member of ETS oncogene family antibody, ETS domain containing protein Elk 1 antibody, ETS domain containing protein Elk1 antibody, ETS domain protein Elk1 antibody, ETS domain-containing protein Elk-1 antibody, ETS like gene 1 antibody, Member of ETS oncogene family antibody, Oncogene Elk1 antibody, Tyrosine kinase (ELK1) oncogene antibody

Gene ID

2002

Swiss Prot

P19419

Modification Site

Ser383

Host

Rabbit

Cross Reactivity

Mouse, Rat

Target

Elk-1 (Ser383)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser383 of human Elk-1, conjugated to keyhole limpet hemocyanin (KLH) .

Applications

Purification

Antigen Affinity purification from Pooled whole antiserum

Concentration

Lot Dependent

Dilution

WB (1:300-5000)

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylation

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

Specific for ~46 kDa Elk-1 phosphorylated at Ser383. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.