DENND3 (Thr450) Antibody
Product Specifications
Background
The DENN (differentially expressed in normal and neoplastic cells) domain (DENND) is a poorly characterized protein module conserved throughout evolution (McPherson et al., 2011) . Proteins bearing a DENN domain have recently emerged as the largest family of Rab GEFs. Among these DENN domain proteins, DENND3 is a GEF for Rab12; promoting the exchange of GDP to GTP, converting inactive GDP-bound RAB12 into its active GTP-bound form (McPherson and Xu, 2015; Matsui et al., 2011) .
Synonyms
DENN Domain Containing 3, DENN Domain-Containing Protein 3, DENN/MADD Domain Containing 3, KIAA0870 DENN/MADD domain containing 3, KIAA0870DENN domain-containing protein 3
Gene ID
315055
Swiss Prot
A2RUS2
Modification Site
Thr450
Host
Rabbit
Cross Reactivity
Human
Target
DENND3 (Thr450)
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Source
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr450 of human DENND3, conjugated to keyhole limpet hemocyanin (KLH) .
Applications
WB
Purification
Antigen Affinity purification from Pooled whole antiserum
Concentration
Lot Dependent
Dilution
WB (1:300-5000)
Buffer
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Modification
Phosphorylation
Storage Conditions
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Specificity
Specific for endogenous levels of the ~142 kDa DENND3 phosphorylated at Thr450. The immunolabeling is completely eliminated by treatment with λ-phosphatase.
Gene ID URL
315055
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