Connexin 43 (Ser368) Antibody

Product Specifications

Background

Gap junctional intercellular communication is thought to play a key role in development and may also be involved in epilepsy (Aronica et al., 2001) . Connexin43 forms gap-junctional channels and regulates the permeability of these gap junctions to small organic molecules. Permeability of connexin43 is known to be regulated by phosphorylation at Ser-368 by protein kinase C (Yogo et al., 2002; Bao et al., 2004a) . Phosphorylation of Ser-368 by PKC induces a conformational change of connexin43 that results in a decrease in gap junction permeability (Bao et al., 2004b) .

Synonyms

Connexin 43 antibody, Connexin-43 antibody, Cx 43 antibody, Cx43 antibody, CXA1_HUMAN antibody, DFNB38 antibody, Gap junction 43 kDa heart protein antibody, Gap junction alpha-1 protein antibody, Gap junction protein alpha 1 43kDa (connexin 43) antibody, Gap junction protein alpha 1 43kDa antibody, Gap junction protein alpha like antibody, GJA 1 antibody, Gja1 antibody, GJAL antibody, ODD antibody, ODDD antibody, ODOD antibody, SDTY3 antibody

Gene ID

24392

Swiss Prot

P08050

Modification Site

Ser368

Host

Rabbit

Cross Reactivity

Mouse, Rat

Target

Connexin 43 (Ser368)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser368 conjugated to keyhole limpet hemocyanin (KLH) .

Applications

Purification

Antigen Affinity purification from Pooled whole antiserum

Concentration

Lot Dependent

Dilution

WB (1:300-5000)

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylation

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

Specific for endogenous levels of the ~43 kDa connexin-43 protein phosphorylated at Ser368. Immunolabeling is completely eliminated by treatment with λ-phosphatase.