Cdc2 (Tyr15) Antibody

Product Specifications

Background

Cdc2 is a highly conserved protein serine kinase that plays a key role in regulation of the cell cycle (Maller, 1991) . The ability of cdc2 to exercise control over the cell cycle is dependent upon the phosphorylation of Tyr-15 in cdc2 (Nakamizo et al., 2002) . cdc2 expression in brain has been linked to both neurogenesis and apoptosis (Konishi and Bonni, 2003; Dranovsky et al., 2001; Okano et al., 1996) .

Synonyms

Cdc 2 antibody, Cdc2 antibody, CDC28A antibody, CDK-1 antibody, CDK 1 antibody, CDK1 antibody, CDK1_HUMAN antibody, CDKN1 antibody, CELL CYCLE CONTROLLER CDC2 antibody, Cell division control protein 2 antibody, Cell division control protein 2 homolog antibody, Cell division cycle 2 G1 to S and G2 to M antibody, Cell division protein kinase 1 antibody, Cell Divsion Cycle 2 Protein antibody, Cyclin Dependent Kinase 1 antibody, Cyclin-dependent kinase 1 antibody, DKFZp686L20222 antibody, MGC111195 antibody, p34 Cdk1 antibody, p34 protein kinase antibody, P34CDC2 antibody

Gene ID

54237

Swiss Prot

P39951

Modification Site

Tyr15

Host

Rabbit

Cross Reactivity

Human, Mouse, Xenopus

Target

Cdc2 (Tyr15)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr15 of rat cdc2, conjugated to keyhole limpet hemocyanin (KLH) .

Applications

Purification

Antigen Affinity purification from Pooled whole antiserum

Concentration

Lot Dependent

Dilution

WB (1:300-5000)

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylation

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

Specific for endogenous levels of the ~34 kDa cdc2 protein phosphorylated at Tyr15. Immunolabeling is completely eliminated by treatment with λ-phosphatase.