What is the catalog number for CaM Kinase II (Thr306) Antibody?

The catalog number for CaM Kinase II (Thr306) Antibody is bs-70031R.

What sizes are available for CaM Kinase II (Thr306) Antibody?

CaM Kinase II (Thr306) Antibody is available in the following size(s): 100 µL.

Is CaM Kinase II (Thr306) Antibody in stock?

CaM Kinase II (Thr306) Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

What expression system was used for this recombinant protein?

The expression system, such as E. coli, HEK293, or insect cells, is listed on the datasheet under production details, which can affect post-translational modifications relevant to your application.

Can I return this product if I ordered the wrong one?

As a consumer, you have a 14-day right of withdrawal from receipt under EU consumer protection law, submitted via our withdrawal/cancellation request form. Note this applies to unused, undamaged products, and doesn’t override the expiry/storage conditions on performance-based claims.

Is this product tested for endotoxin levels?

Endotoxin testing status is noted on the datasheet where applicable, particularly for recombinant proteins intended for cell-based or functional assays. If this isn’t listed and you need confirmation, contact support with the catalogue number.

CaM Kinase II (Thr306) Antibody

Product Specifications

Background

Ca2+/Calmodulin-Dependent Protein Kinase II (CaM Kinase II) is a multifunctional calcium and calmodulin-dependent protein kinase that mediates cellular responses to a wide variety of intercellular signals (Kennedy, 1998; Schulman and Hanson, 1993) . CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+-homeostasis (Gleason et al., 2003; Soderling, 2000; Hudmon and Schulman, 2002) . Phosphorylation of Thr-286 on the kinase produces an autonomously active form of CaM Kinase II (Meng et al., 2003; Picciotto et al., 1993) . CaMKIIα autophosphorylation at Thr-286 and Thr-305/Thr-306 has recently been shown to regulate kinase activity and modulate subcellular targeting and is critical for normal synaptic plasticity and learning and memory (Baucum et al., 2015) .

Synonyms

Calcium/calmodulin dependent protein kinase II alpha antibody, Calcium/calmodulin dependent protein kinase II beta antibody, Calcium/calmodulin dependent protein kinase II delta antibody, Calcium/calmodulin dependent protein kinase II gamma antibody, Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody, CaM kinase II alpha antibody, CaM kinase II antibody, CaM kinase II beta antibody, CaM kinase II delta antibody, CaM kinase II gamma antibody, CaM kinase II subunit alpha antibody, CaMK-II subunit alpha antibody, CAMK2 antibody, Camk2a antibody, CAMK2B antibody, CAMK2D antibody, CAMK2G antibody, CAMKA antibody, KCC2A_HUMAN antibody

Gene ID

25400

Swiss Prot

P11310

Modification Site

Thr306

Host

Rabbit

Cross Reactivity

Mouse, Rat

Target

CaM Kinase II (Thr306)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr306 of rat CaMKII, conjugated to keyhole limpet hemocyanin (KLH) .

Applications

Purification

Antigen Affinity purification from Pooled whole antiserum

Concentration

Lot Dependent

Dilution

WB (1:300-5000)

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylation

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

Specific for endogenous levels of the ~50 kDa α-CaM Kinase II and the ~60 kDa β-CaM Kinase II proteins phosphorylated at Thr306. Immunolabeling is completely eliminated by treatment with λ-phosphatase. It has been reported that this antibody may recognize some level of non-phosphorylated pure recombinant protein, but in our hands (as shown in our WB image) in native tissue, the antibody reacts in a phospho-specific manner.