Calretinin Antibody

Product Specifications

Background

Calretinin is a calcium-binding protein involved in calcium signaling. It is abundantly expressed throughout the central nervous system, retina and many other tissues. Studies have shown that calretinin may play a role in neuroprotection by buffering intracellular calcium (Lukas and Jones 1994, Weisenhorn et al., 1996) . Additionally, calretinin selectively stains mesothelial cells thus making it an important marker for differentiating malignant mesothelioma from carcinomas (Attanoos et al., 2000) .

Synonyms

29 kDa calbindin antibody, CAB 29 antibody, CAB29 antibody, CAL 2 antibody, CAL2 antibody, CALB 2 antibody, CALB2 antibody, CALB2_HUMAN antibody, Calbindin 2 29kDa antibody, Calbindin 2 antibody, Calbindin D29K antibody, Calbindin2 antibody, Calretinin antibody, CR antibody

Gene ID

117059

Swiss Prot

P47728

Host

Sheep

Cross Reactivity

Rat

Target

Calretinin

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

Purified guinea pig calretinin.

Applications

WB, IHC

Purification

Purified by Protein G.

Concentration

Lot Dependent

Dilution

WB (1:300-5000), IHC ()

Buffer

10 mM Tris, 100 mM NaCl

Modification

Unmodified

Storage Conditions

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Specificity

Specific for endogenous levels of the ~29 kDa calretinin protein in Western blots of rat brain lysate. The antibody also works well for immunohistochemistry on formalin or paraformaldehyde perfused rat brain tissue sections.