Beta Catenin Antibody

Product Specifications

Background

Beta Catenin, considered a moonlighting protein, is a component of mammalian cell adhesion complex in the cytoplasm along with playing a key downstream component of the canonical Wingless Int-1 (Wnt) signaling pathway in the nucleus (McTrea P.D et al, 1991 and Kemler R. et al, 1993) . As part of a protein complex that forms adheren junctions, beta Catenin is necessary for the creation and maintenance of epithelial cell layers and barriers (Brembeck F.H. et al, 2006) . beta Catenin is responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete (Brembeck F.H. et al, 2006) . The Wnt/beta Catenin signaling pathway plays important roles in mammalian development (Logan C.Y. et al, 2004) and irregular regulation of this signaling pathway is associated with human diseases and cancers (Moon R.T., 2004) . HIPK2 is a negative regulator of the Wnt signaling via the direct phosphorylation of beta Catenin at ser33/37 causing degradation (Kim E.A. et al, 2010) . Additionally, stabilization of beta Catenin has been shown when AMPK phosphorylates at ser552 regulating energy metabolism to cell differentiation and development by enhancing the beta Catenin/TCF mediated transcription within the Wnt/betaCatenin signaling pathway (Zhao J. et al, 2010) .

Synonyms

B-catenin antibody, Beta catenin antibody, Beta-catenin antibody, Cadherin associated protein antibody, Catenin (cadherin associated protein), beta 1, 88kDa antibody, Catenin beta 1 antibody, Catenin beta-1 antibody, CATNB antibody, CHBCAT antibody, CTNB1_HUMAN antibody, CTNNB antibody, CTNNB1 antibody, DKFZp686D02253 antibody, FLJ25606 antibody, FLJ37923 antibody, OTTHUMP00000162082 antibody, OTTHUMP00000165222 antibody, OTTHUMP00000165223 antibody, OTTHUMP00000209288 antibody, OTTHUMP00000209289 antibody

Gene ID

84353

Swiss Prot

Q9WU82

Host

Rabbit

Cross Reactivity

Mouse, Rat

Target

Beta Catenin

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

Synthetic peptide corresponding to amino acid residues from the C-terminal region of rat beta Catenin, conjugated to keyhole limpet hemocyanin (KLH) .

Applications

Purification

Antigen Affinity purification from Pooled whole antiserum

Concentration

Lot Dependent

Dilution

WB (1:300-5000)

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Unmodified

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

Specific for endogenous levels of the ~ 92 kDa beta Catenin protein. Immunolabeling of the beta Catenin band is completely blocked by preadsorption of the antibody with the peptide used as antigen.