ATRIP (Ser239) Antibody

Product Specifications

Background

ATRIP, ATR interacting protein, binds to ATR to regulate ATR expression, and is an essential component of the DNA damage checkpoint pathway (Cortez et al, 2001) . ATR is recruited to DNA lesions in part through its association with ATRIP, which in turn interacts with the single-stranded DNA binding protein RPA (Ball et al, 2007) . DNA replication forks may stall as a result of DNA damage causing phosphorylation of several proteins, including BRCA1 when colocalizing with ATR/ATRIP complex and RPA (Venere et al, 2007) . The DNA replication fork stall coincides with BRCA1 directly phosphorylating ATRIP at Ser-239 (Venere et al, 2007) .

Synonyms

AGS1 antibody, ATIP antibody, ATM and Rad3 related interacting protein antibody, ATM and Rad3-related-interacting protein antibody, ATR interacting protein antibody, ATR-interacting protein antibody, Atrip antibody, ATRIP_HUMAN antibody, DKFZp762J2115 antibody, FLJ12343 antibody, MGC20625 antibody, MGC21482 antibody, MGC26740 antibody

Gene ID

84126

Swiss Prot

Q8WXE1

Modification Site

Ser239

Host

Rabbit

Cross Reactivity

Mouse

Target

ATRIP (Ser239)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser239 of human ATRIP, conjugated to keyhole limpet hemocyanin (KLH) .

Applications

Purification

Antigen Affinity purification from Pooled whole antiserum

Concentration

Lot Dependent

Dilution

WB (1:300-5000)

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylation

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

Specific for endogenous levels of the ~86 kDa ATRIP protein phosphorylated at Ser239. The antibody also recognizes a band at ~32 kDa.

Gene ID URL

84126

Predicted Cross Reactivity

Human, Bovine, Sheep, Non-Human Primate

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