WT1 Polyclonal Antibody, AbBy Fluor™ 594 Conjugated
Product Specifications
Background
Transcription factor that plays an important role in cellular development and cell survival. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA.
Synonyms
GUD; AWT1; WAGR; WT33; NPHS4; WIT-2; EWS-WT1; Wilms tumor protein; WT1
Gene ID
7490
Swiss Prot
P19544
Cellular Locus
Cytoplasm, Nucleus
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat
Immunogen
301-400/449
Target
WT1
Clonality
Polyclonal
Isotype
IgG
Conjugation
AbBy Fluor™ 594
Source
KLH conjugated synthetic peptide derived from human Wilms Tumor Protein
Applications
WB, FCM, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
590nm/617nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), FCM (1:20-100), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
7490
Predicted Cross Reactivity
Dog, Cow, Sheep, Pig, Chicken
Available Sizes
Curated Selection
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