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Vinculin Polyclonal Antibody, Cy5.5 Conjugated

Product Specifications

Background

Actin filament (F-actin) -binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.

Synonyms

MV; MVCL; CMD1W; CMH15; HEL114; Vinculin; Metavinculin; VCL

Gene ID

7414

Swiss Prot

P18206

Cellular Locus

Cytoplasm, Cell membrane

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat

Immunogen

1001-1134/1134

Target

Vinculin

Clonality

Polyclonal

Isotype

IgG

Conjugation

Cy5.5

Source

KLH conjugated synthetic peptide derived from human Vinculin

Applications

WB, FCM, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

675nm/694nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), FCM (1:20-100), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Unmodified

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

7414

Predicted Cross Reactivity

Dog, Cow, Pig, Horse, Chicken, Rabbit

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