Vinculin Polyclonal Antibody, AbBy Fluor™ 647 Conjugated
Product Specifications
Background
Actin filament (F-actin) -binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.
Synonyms
MV; MVCL; CMD1W; CMH15; HEL114; Vinculin; Metavinculin; VCL
Gene ID
7414
Swiss Prot
P18206
Cellular Locus
Cytoplasm, Cell membrane
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat
Immunogen
1001-1134/1134
Target
Vinculin
Clonality
Polyclonal
Isotype
IgG
Conjugation
AbBy Fluor™ 647
Source
KLH conjugated synthetic peptide derived from human Vinculin
Applications
WB, FCM, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
650nm/665nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), FCM (1:20-100), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
7414
Predicted Cross Reactivity
Dog, Cow, Pig, Horse, Chicken, Rabbit
Curated Selection
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