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Phospho-BCAR1 (Tyr410) Polyclonal Antibody, HRP Conjugated

Product Specifications

Background

Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.

Synonyms

CAS; CAS1; CASS1; CRKAS; P130Cas; Breast cancer anti-estrogen resistance protein 1; CRK-associated substrate; Cas scaffolding protein family member 1; BCAR1

Gene ID

9564

Swiss Prot

P56945

Modification Site

Tyr410

Cellular Locus

Cytoplasm, Cell membrane

Host

Rabbit

Cross Reactivity

Rat

Target

Phospho-BCAR1 (Tyr410)

Clonality

Polyclonal

Isotype

IgG

Conjugation

HRP

Source

KLH conjugated synthetic phosphopeptide derived from human BCAR1 around the phosphorylation site of Tyr410.

Applications

ELISA, IHC-P, IHC-F

Purification

Purified by Protein A.

Concentration

1µg/µl

Dilution

ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Specificity

Will recognize BCAR1 (Tyr414) in mouse and BCAR1 (Tyr508) in rat

Gene ID URL

9564

Predicted Cross Reactivity

Human, Mouse, Horse

Curated Selection

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