Adenovirus 5 E1A Polyclonal Antibody, RBITC Conjugated
Product Specifications
Background
The early region (E1) of the adenovirus genome, responsible for transforming activity, is localized within the left most 11% of the viral genome and consists of two transcriptional units E1A and E1B. E1A is sufficient for partial transformation and immortalization of primary cells. E1A gene products are necessary for normal levels of transcription of the other early regions of the adenovirus genome during productive infection and are able to either activate or repress the transcription of specific cellular genes. E1A forms specific complexes with cellular proteins including p105 causing inhibition of the cell cycle inducing arresting function of p105.
Synonyms
Adenovirus early E1A protein; Control protein E1A; E1A; Early E1A protein; Human adenovirus type 5; Human adenovirus C; pfam02703; E1A_ADE05.
Swiss Prot
P03255
Cellular Locus
Cytoplasm, Nucleus, Extracellular matrix
Host
Rabbit
Cross Reactivity
Virus
Immunogen
181-280/289
Target
Adenovirus 5 E1A
Clonality
Polyclonal
Isotype
IgG
Conjugation
RBITC
Source
KLH conjugated synthetic peptide derived from human Adenovirus 5 E1A
Applications
WB, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
570nm/595nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Cross Reactivity Details
Adenovirus5
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