Phospho-ROCK2 (Tyr256) Polyclonal Antibody
Product Specifications
Background
The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.
Synonyms
ROCK2phospho Y256; p164 ROCK 2; Rho associated coiled coil containing protein kinase 2; Rho associated protein kinase 2; Rho kinase 2; ROCK 2; Rock II; Rock2m; ROK alpha; ROKalpha; ROCK2_HUMAN.
Gene ID
9475
Modification Site
Tyr256
Cellular Locus
Cytoplasm, Nucleus, Cell membrane
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat
Target
Phospho-ROCK2 (Tyr256)
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Source
KLH conjugated synthetic phosphopeptide derived from human ROCK2 around the phosphorylation site of Tyr256
Applications
ELISA, FCM, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Concentration
1µg/µl
Dilution
ELISA (1:500-1000), FCM (1:20-100), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Gene ID URL
9475
Predicted Cross Reactivity
Dog, Cow, Pig, GuineaPig
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