Phospho-ROCK2 (Thr249) Polyclonal Antibody, PE Conjugated
Product Specifications
Background
The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.
Synonyms
ROCK2phospho T249; p164 ROCK 2; Rho associated coiled coil containing protein kinase 2; Rho associated protein kinase 2; Rho kinase 2; ROCK 2; Rock II; Rock2m; ROK alpha; ROKalpha; ROCK2_HUMAN.
Gene ID
9475
Modification Site
Thr249
Cellular Locus
Cytoplasm, Nucleus, Cell membrane
Host
Rabbit
Cross Reactivity
Human
Target
Phospho-ROCK2 (Thr249)
Clonality
Polyclonal
Isotype
IgG
Conjugation
PE
Source
KLH conjugated synthetic phosphopeptide derived from human ROCK2 around the phosphorylation site of Thr249
Applications
WB, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
496,564nm/578nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
9475
Predicted Cross Reactivity
Mouse, Rat, Dog, Cow, Pig, Horse, Rabbit, GuineaPig
Available Sizes
Curated Selection
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