Phospho-YES1 (Tyr426) Polyclonal Antibody, APC-Cy7 Conjugated

Product Specifications

Background

Non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation. Stimulation by receptor tyrosine kinases (RTKs) including EGRF, PDGFR, CSF1R and FGFR leads to recruitment of YES1 to the phosphorylated receptor, and activation and phosphorylation of downstream substrates. Upon EGFR activation, promotes the phosphorylation of PARD3 to favor epithelial tight junction assembly. Participates in the phosphorylation of specific junctional components such as CTNND1 by stimulating the FYN and FER tyrosine kinases at cell-cell contacts. Upon T-cell stimulation by CXCL12, phosphorylates collapsin response mediator protein 2/DPYSL2 and induces T-cell migration. Participates in CD95L/FASLG signaling pathway and mediates AKT-mediated cell migration. Plays a role in cell cycle progression by phosphorylating the cyclin-dependent kinase 4/CDK4 thus regulating the G1 phase. Also involved in G2/M progression and cytokinesis.

Synonyms

Yes; c-yes; HsT441; P61-YES; Tyrosine-protein kinase Yes; Proto-oncogene c-Yes; YES1

Gene ID

7525

Swiss Prot

P07947

Modification Site

Tyr426

Cellular Locus

Cytoplasm

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat

Immunogen

420-430/543

Target

Phospho-YES1 (Tyr426)

Clonality

Polyclonal

Isotype

IgG

Conjugation

APC-Cy7

Source

KLH conjugated synthetic phosphopeptide derived from human YES1 around the phosphorylation site of Tyr426

Applications

WB, IF (IHC-P)

Purification

Purified by Protein A.

Excitation Emission

650nm/780nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), IF (IHC-P) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Specificity

Due to the highly conserved nature of this protein, this antibody will react with the related proteins LCK (Tyr394), Src (Tyr418), and Lyn (Tyr397) if phosphorylated at this site due to homology.