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Phospho-PI3KCA (Tyr317) Polyclonal Antibody, FITC Conjugated

Product Specifications

Background

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.

Synonyms

PEPCK; PEPCK2; PEPCK-M; Phosphoenolpyruvate carboxykinase [GTP], mitochondrial; PCK2

Gene ID

5106

Swiss Prot

Q16822

Modification Site

Tyr317

Cellular Locus

Cytoplasm, Cell membrane

Host

Rabbit

Cross Reactivity

Human, Mouse

Target

Phospho-PI3KCA (Tyr317)

Clonality

Polyclonal

Isotype

IgG

Conjugation

FITC

Source

KLH conjugated synthetic phosphopeptide derived from human PI3KCA around the phosphorylation site of Tyr317

Applications

WB, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

494nm/518nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

5106

Predicted Cross Reactivity

Rat

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