Phospho-PI3KCA (Tyr317) Polyclonal Antibody, Cy3 Conjugated
Product Specifications
Background
Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
Synonyms
PEPCK; PEPCK2; PEPCK-M; Phosphoenolpyruvate carboxykinase [GTP], mitochondrial; PCK2
Gene ID
5106
Swiss Prot
Q16822
Modification Site
Tyr317
Cellular Locus
Cytoplasm, Cell membrane
Host
Rabbit
Cross Reactivity
Human, Mouse
Target
Phospho-PI3KCA (Tyr317)
Clonality
Polyclonal
Isotype
IgG
Conjugation
Cy3
Source
KLH conjugated synthetic phosphopeptide derived from human PI3KCA around the phosphorylation site of Tyr317
Applications
WB, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
512,550nm/570,615nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
5106
Predicted Cross Reactivity
Rat
Curated Selection
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