Phospho-AMPK alpha-1 (Thr183) Polyclonal Antibody, Cy7 Conjugated
Product Specifications
Background
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK) . AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq].
Synonyms
AMPK alpha 1 + AMPK alpha 2 phospho S485 + S491; PRKAA1phospho S79; 5 AMP activated protein kinase alpha 1catalytic subunit; 5 AMP activated protein kinase catalytic alpha 1 chain; 5' AMP activated protein kinase catalytic subunit alpha 1; AAPK1; acetyl CoA carboxylase kinase; AI194361 antibody AI450832 ; AL024255 ; AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase, catalytic, alpha -1; AMPK 1; AMPK alpha 1 chain; AMPK antibody AMPK1; AMPKa1; AMPKalpha1; C130083N04Rik; cb116 antibody EC 2.7.11.1; HMG CoA reductase kinase; hormone sensitive lipase kinase; im:7154392 antibody kinase AMPK alpha1 ; MGC33776; MGC57364 antibody PRKAA 1; PRKAA1; Protein kinase AMP activated alpha 1 catalytic subunit; SNF1-like protein AMPK; wu:fa94C10.
Gene ID
5562
Modification Site
Thr198
Cellular Locus
Cytoplasm, Nucleus
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat
Target
Phospho-AMPK alpha-1 (Thr183)
Clonality
Polyclonal
Isotype
IgG
Conjugation
Cy7
Source
KLH conjugated synthetic phosphopeptide derived from human PRKAA1 around the phosphorylation site of Thr198
Applications
WB, FCM, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
743nm/767nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), FCM (1:20-100), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
5562
Predicted Cross Reactivity
Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit
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