Phospho-MAPK8 (Thr183 + Tyr185) Polyclonal Antibody, RBITC Conjugated
Product Specifications
Background
Phosphorylated at the Thr-Pro-Tyr phosphorylation motif instead of the characteristic MAP kinase Thr-Glu-Tyr motif. JNK2 (p54a, SAPK1a), along with JNK1 and JNK3, is thought to play an important role in nuclear signal transduction through its environmental stress activation and subsequent phosphorylation of the nuclear transcription factor p53.
Synonyms
MAPK8/MAPK9phospho T183/Y185; Mapk9; C Jun kinase 2; C Jun N terminal kinase 2; JNK 2; JNK-2; JNK 55; JNK2 alpha; JNK2 beta; JNK2alpha; JNK2B; JNK2beta; JNK55; Jun kinase; MAP kinase 9; MAPK 9; Mitogen activated protein kinase 9; P54 alpha; P54aSAPK; PRKM9; SAPK alpha; SAPK1a; SAPKa; Stress activated protein kinase JNK2.
Gene ID
5601
Modification Site
Thr183 + Tyr185
Cellular Locus
Nucleus
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat
Target
Phospho-MAPK8 (Thr183 + Tyr185)
Clonality
Polyclonal
Isotype
IgG
Conjugation
RBITC
Source
KLH conjugated synthetic phosphopeptide derived from human MAPK9 around the phosphorylation site of Thr183+Tyr185
Applications
WB, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
570nm/595nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
5601
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