Phospho-ERK5 (Ser731+Thr733) Polyclonal Antibody, Cy5 Conjugated
Product Specifications
Background
Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression. Involved in the regulation of p53/TP53 by disrupting the PML-MDM2 interaction.
Synonyms
BMK1; ERK4; ERK5; PRKM7; Mitogen-activated protein kinase 7; MAP kinase 7; MAPK 7; Big MAP kinase 1; BMK-1; Extracellular signal-regulated kinase 5; ERK-5; MAPK7
Gene ID
5598
Swiss Prot
Q13164
Modification Site
Ser731+Thr733
Cellular Locus
Cytoplasm, Nucleus
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat
Target
Phospho-ERK5 (Ser731+Thr733)
Clonality
Polyclonal
Isotype
IgG
Conjugation
Cy5
Source
KLH conjugated synthetic phosphopeptide derived from human ERK5 around the phosphorylation sites of Ser731+Thr733
Applications
WB, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
625,650nm/670nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Specificity
Recognizes Ser721+Thr723 in mice and rats
Gene ID URL
5598
Predicted Cross Reactivity
Dog, Cow, Rabbit
Curated Selection
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