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Phospho-JunD (Ser255) Polyclonal Antibody, AbBy Fluor™ 647 Conjugated

Product Specifications

Background

The cJun proto-oncogene was first identified as the cellular homolog of the avian sarcoma virus vjun oncogene. JunB and JunD have been shown to be almost identical to cJun in their C terminal regions, which are involved in dimerization and DNA binding, whereas their N terminal domains, which are involved in transcriptional activation, diverge. JunB is a transcription factor involved in regulating gene activity following the primary growth factor response. It binds to the DNA sequence 5'-TGA[CG]TCA-3'.

Synonyms

AP-1; Transcription factor jun-D; JUND

Gene ID

3727

Swiss Prot

P17535

Modification Site

Ser255

Cellular Locus

Nucleus

Host

Rabbit

Cross Reactivity

Human

Target

Phospho-JunD (Ser255)

Clonality

Polyclonal

Isotype

IgG

Conjugation

AbBy Fluor™ 647

Source

KLH conjugated synthetic phosphopeptide derived from human JunD around the phosphorylation site of Ser255

Applications

WB, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

650nm/665nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

3727

Predicted Cross Reactivity

Dog, Cow, Chicken

Curated Selection

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