Phospho-JunB (Ser259) Polyclonal Antibody

Product Specifications

Background

The cJun proto-oncogene was first identified as the cellular homolog of the avian sarcoma virus vjun oncogene. JunB and JunD have been shown to be almost identical to cJun in their C terminal regions, which are involved in dimerization and DNA binding, whereas their N terminal domains, which are involved in transcriptional activation, diverge. JunB is a transcription factor involved in regulating gene activity following the primary growth factor response. It binds to the DNA sequence 5'-TGA[CG]TCA-3'.

Synonyms

Activator protein 1; AP 1; AP1; Jun B; Jun B proto oncogene; Jun B protooncogene; JunB proto oncogene; JunB protoncogene 9; JunB protooncogene; Transcription factor jun B; Transcription factor junB.

Gene ID

3726

Modification Site

Ser259

Cellular Locus

Nucleus

Host

Rabbit

Target

Phospho-JunB (Ser259)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

KLH conjugated synthetic phosphopeptide derived from human JunB around the phosphorylation site of Ser259 [PV (p-S) PI]

Applications

WB, ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Concentration

1µg/µl

Dilution

WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Gene ID URL

3726

Predicted Cross Reactivity

Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Guinea Pig

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