Phospho-ILK-1 (Ser246) Polyclonal Antibody, Cy3 Conjugated
Product Specifications
Background
The ILK protein is important in different biological pathways such as cell adhesion, anchorage-dependent cell cycle progression, oncogenic transformation, and growth factor signaling. The kinase activity of ILK is low in non-activated cells; its activity is stimulated by cell-ECM interactions and by certain growth factors. 3 Negative regulation of ILK is mediated by two phosphatases: PTEN, a tumor suppressor lipid sphatase, and ILKAP, a PP2C protein phosphatase. In tumor cells that do not express PTEN protein, ILK is constitutively active.
Synonyms
ILK-1 phospho S246; ILK-1 phospho Ser246; p-ILK-1 Ser246; 59 kDa serine/threonine protein kinase; 59 kDa serine/threonine-protein kinase; ILK-2; ILK_HUMAN; Integrin linked Kinase; Integrin-linked protein kinase; DKFZp686F1765; EC 2.7.11.1; ILK 1; ILK 2; ILK; ILK1; ILK2; Integrin linked Kinase 2; Integrin linked protein kinase; p59; p59ILK.
Gene ID
3611
Modification Site
Ser246
Cellular Locus
Cytoplasm, Cell membrane
Host
Rabbit
Cross Reactivity
Human, Mouse
Target
Phospho-ILK-1 (Ser246)
Clonality
Polyclonal
Isotype
IgG
Conjugation
Cy3
Source
KLH conjugated synthetic phosphopeptide derived from human ILK-1 around the phosphorylation site of Ser246
Applications
WB, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
512,550nm/570,615nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
3611
Predicted Cross Reactivity
Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit
Curated Selection
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